It’s been a while since the last one of these, so here are listed 10 frequently asked questions about the Labrox 5in1 Multilabel Plate Reader. If you have any other concerns, please don’t hesitate to contact us via e-mail: firstname.lastname@example.org
- How do I add a new optical filter into the Labrox 5in1 multilabel reader? Do I have to update the software after the filter addition?
A new optical filter is added very easily. Go to the “Optics” page of the software, click the “Create New” -button and name the new filter. Click the “Load Filter” -button, then the filter storage will be open automatically and you can add the new filter into the storage system. Click the “Done” -button — then Labrox 5in1 multilabel plate reader automatically storages the filter into the new place. You do not have to update the software as it recognizes the filter automatically the next time when the software is opened.
2. How I stop the assay during the run?
The assay can be stopped during the run just by clicking the “Break Measurement” -button. If the reader has measured some wells before stopping the assay, results are seen and can be exported. The assay can be started again by clicking the “Start Measurement” -button.
3. Can I measure absorbance using two absorbance wavelengths at the same time?
We have two options for absorbance measurements. With a filter-based absorbance unit, only one wavelength at a time is measured. With a spectrometer-based absorbance unit, you can choose as many absorbance wavelengths as you want. In spectrometer-based absorbance assays, the instrument measures the whole absorbance spectrum from 220nm to 1000nm with 1nm increasements.
4. What kind of plates can be used in the Labrox 5in1 multimodal plate reader?
All kinds of plates can be used in the reader, from 6-well plates to 1536-well plates. Black, white and transparent plates can be used depending on the detection mode. If there is not a suitable plate at the software library, it is easy to add a new plate into the library using the Plate Wizard -program.
5. How many optical filters can be added to the filter storage system?
There are 32 filter place positions available on the filter storage system. Filter storage keeps the optical filters safe from dust and loss. Same optical filters can be used for the bottom and top readings, then there is no need to use extra filters.
6. I want to do my own protocol, how I save it?
After you have chosen all parameters, you can save the protocol just by clicking the “Save” -button,and then the protocol is ready to use. The created protocol is shown in the protocol list.
7. Can I read from the bottom of the plate?
Yes, you can read from the bottom of the plate. The bottom reading can be chosen when the protocol is made. The bottom reading is suitable such as for cell applications.
8. What is the time for reading the whole 96-well plate? And what is the time for reading the whole 384-well plate?
Measuring time depends on the number of flashes used in the protocol. For example, when the flash amount is 50, the measuring time for 96-well plate is one minute and 30 seconds, and for 384-well plate, 5 minutes and 20 seconds, respectively.
9. Can I shake and incubate the plate at +37 ºC before measuring?
The plate can be shaken and incubated before measuring. The shaking can be done in three different ways; linear, orbital and double orbital shaking. The shaking speed and interval can be also chosen. Temperature control can be chosen between ambient +3 ºC to +65 ºC. Temperature control can be on while measuring the plate.
10. What is the minimum dispensing volume when using Labrox 5in1 multilabel reader’s dispenser?
The minimum dispensing volume is 5µl and the dispenser can dispense up to 5ml. Labrox 5in1 multilabel reader can be equipped with one or two dispensers.